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目的对已成功制备的以脱细胞犬动脉为基质的血管支架进行体外再细胞化研究。方法多聚环氧化合物家族的乙二醇缩水甘油醚(ethylene glycol diglycidyl ether,EX-810)、去离子水和超声共同作用于犬主动脉,制备血管支架,并在体外对其进行有效的再细胞化,包括人脐动脉平滑肌细胞(smooth muscle cell,SMC)的种植,人脐静脉内皮细胞(endothelial cell,EC)的种植,以及两种细胞的复合种植。结果本文提出的制备血管支架适合SMC和EC的生长,以合适的接种密度进行再细胞化即可获得致密的细胞层;提高细胞的种植密度可以加速支架的再细胞化进程。SMC和EC复合种植模型中,高密度接种的EC能够在不同密度的SMC上生长,形成较为致密的EC单层;但共培养的EC的形态和覆盖率受SMC接种密度的影响。结论在脱细胞犬动脉上成功实现EC和SMC的单独种植和联合种植。
Abstract：Objective To recellularize a novel tissue engineered blood vessel (TEBV) scaffold based on decellularized canine aorta which we had successfully constructed. Methods Canine aortas were decellularized under the co-action of EX-810, ion-free water, and ultrasound. Then this kind of scaffolds was recellularized in vitro, including the seeding of smooth muscle cells (SMC), seeding of endothelial cells (EC), and dual seeding of these two kinds of cells. Results The TEBV scaffolds we had constructed were suitable for SMC and EC to grow on; a nearly confluent layer of either SMC or EC could be achieved when seeded in proper density. The recellularization process was accelerated by increasing the seeding density. In the dual seeding system, EC could grow well directly on the layer of SMC with different seeding density, while the morphology and covering rate of EC were influenced by the density of their co-culture SMC. Conclusion SMC and EC can be successfully seeded on the decellularized canine aortas separately or unitedly.
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基本信息:
DOI：10.16289/j.cnki.1002-0837.2007.05.003
中图分类号:R318.1
引用信息:
[1]涂秋芬,张怡,吴江等.以脱细胞犬动脉为基质的血管支架体外再细胞化[J].航天医学与医学工程,2007(05):358-363.DOI:10.16289/j.cnki.1002-0837.2007.05.003.
基金信息:
国家211工程项目“生物医学材料与人工器官”资助