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目的探讨红益胶囊对-10°头低位卧床28 d诱导的猕猴下肢骨骼肌萎缩的保护作用,开发红益胶囊在肌萎缩防护中的新用途。方法采用-10°头低位卧床制备猕猴模拟失重性肌萎缩模型;猕猴分为对照组和喂食红益胶囊组,分别在卧床前(0天)和卧床后28 d取一侧比目鱼肌,应用免疫组织化学和实时定量PCR等方法,分析红益胶囊对肌萎缩形成的影响。结果 1)卧床28 d后,猕猴小腿平均围径和比目鱼肌肌纤维平均直径较卧床前显著下降(P<0.05),直径较小肌纤维含量明显增多,提示-10°头低位卧床28 d明显诱导了猕猴下肢骨骼肌和肌纤维的萎缩。2)卧床28 d后,猕猴比目鱼肌肌纤维横截面积明显下降,但红益胶囊组下降幅度较对照组显著减少(P<0.01)。3)-10°头低位卧床28 d后,肌萎缩特异因子Atrogin-1 mRNA的表达显著增加,红益胶囊组Atrogin-1 mRNA的表达受到了明显抑制(P<0.05)。结论红益胶囊可通过抑制肌萎缩特异因子Atrogin-1 mRNA的表达对抗-10°头低位卧床诱导的肌萎缩。提示了红益胶囊在肌萎缩防护中的新功能。
Abstract：Objective To explore the effects of Hongyi capsules on rhesus monkey limb skeletal muscle atrophy induced by- 10° head down bed rest( HDBR) for 28 d,and develop new usage of Hongyi capsules in protecting against muscle atrophy. Methods Rhesus monkey were divided into control and Hongyi capsules-treated group and fixed to bed in- 10°HDBR to prepare simulated weightlessness induced muscular atrophy model. One side of the soleus muscle were removed at 0 and the 28th day of- 10° HDBR respectively,then detected the effect of Hongyi capsules on muscle atrophy by immunohistochemistry and real time PCR. Results 1) After 28 d of- 10° HDBR,the average calf circumference and diameter of soleus fibers were decreased significantly( P <0. 05),and the distribution of muscle fibers showed significant increase of smaller fibers. It was demonstrated that- 10° HDBR for 28 days significantly induced skeletal muscle fiber atrophy. 2) After 28 days of- 10° HDBR,the cross-sectional area( CSA) of soleus muscle fiber from both groups were decreased,but the decrease was inhibited significantly in Hongyi capsules-treated group( P < 0. 01),which indicated that Hongyi capsules could effectively inhibit the decline of muscle fiber cross-sectional area during- 10° HDBR-induced muscle atrophy. 3) After 28 days of- 10° HDBR,Atrogin-1 mRNA expression was increased in both groups, but was notably inhibited in Hongyi capsules-treated group compared with control group( P < 0. 05). Conclusion Hongyi capsules can resist muscular atrophy by inhibiting the expression of Atrogin-1 mRNA expression during- 10° HDBR-induced muscle atrophy,which provides the new function of Hongyi capsules in protecting against muscle atrophy.
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基本信息:
DOI：10.16289/j.cnki.1002-0837.2013.06.004
中图分类号:R852.22
引用信息:
[1]罗维,张鹏,李文炯,等.红益胶囊对猕猴28天头低位卧床肌萎缩的保护作用[J].航天医学与医学工程,2013,26(06):455-458.DOI:10.16289/j.cnki.1002-0837.2013.06.004.
基金信息:
国家自然科学基金面上项目(31171144,81272177);; 航天医学基础与应用国家重点实验室研究基金(SMFA10A01,SMFA13A01);; 中国航天医学工程预先研究项目(2012SY54A1601)