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目的 采用网络药理学及分子对接技术，探索红景天降低颅内高压、保护航天员视力的关键靶点及相关作用机制。方法 通过筛选红景天与颅内高压的共同靶点，绘制“中药-化学成分-共同靶点”网络图，构建蛋白质-蛋白质相互作用网络等方法寻找红景天治疗颅内高压的有效成分和关键靶点；通过功能富集分析，探索红景天治疗颅内高压的相关通路；对有效成分与关键靶点进行分子对接，初步验证红景天治疗颅内高压的分子机制。结果 红景天治疗颅内高压的有效成分为山奈酚、没食子酸、7-羟基香豆素、红景天苷；关键靶点为丝氨酸/苏氨酸激酶1(Akt1)、肿瘤抑制蛋白P53(TP53)、β-肌动蛋白（ACTB）、核因子κB1(NF-κB1)、HSP90AA1，得到848条功能条目和152条相关通路。分子对接结果显示：没食子酸与TP53、山奈酚与Akt1以及7-羟基香豆素与Akt1可良好结合。结论 红景天可能通过山奈酚、没食子酸、7-羟基香豆素、红景天苷多种有效成分调控Akt1、TP53、ACTB、NF-κB1、HSP90AA1关键靶点，影响PI3K/Akt1、NF-κB信号通路，在失重状态下发挥降低颅内高压、保护航天员视力的作用。
Abstract：Objective To explore the key targets and underlying mechanisms of Rhodiola rosea in alleviating intracranial hypertension and protecting astronauts' vision using network pharmacology and molecular docking techniques. Methods Common targets between Rhodiola rosea and intracranial hypertension were screened to construct a “Herb-Chemical Component-Common Target” network and a protein-protein interaction(PPI) network, identifying active components and core targets of Rhodiola rosea for treating intracranial hypertension. Functional enrichment analysis was performed to elucidate associated biological pathways. Molecular docking was conducted to validate the binding affinity between active components and key targets. Results The active components of Rhodiola rosea against intracranial hypertension were identified as kaempferol, gallic acid, 7-hydroxycoumarin, and salidroside. The core targets included serine/threonine kinase 1(Akt1), tumor protein P53(TP53), beta-actin(ACTB), nuclear factor kappa B1(NF-κB1), and HSP90 AA1. Functional enrichment analysis determined 848 functional terms and 152 related pathways. Molecular docking results indicated strong binding affinities between gallic acid and TP53, kaempferol and Akt1, and 7-hydroxycoumarin with Akt1. Conclusion Rhodiola rosea may alleviate intracranial hypertension and protect astronaut vision under microgravity conditions by modulating the key targets Akt1, TP53, ACTB, NF-κB1, and HSP90 AA1 through its active components—kaempferol, gallic acid, 7-hydroxycoumarin, and salidroside, thereby influencing the PI3K/Akt1 and NF-κB signaling pathways.
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基本信息:
DOI：10.16289/j.cnki.1002-0837.2026.01001
中图分类号:R856.2
引用信息:
[1]崔德蓉,郭海燕.基于网络药理学与分子对接技术探讨红景天改善航天微重力状态下颅内高压的作用机制[J].航天医学与医学工程,2026,37(01):1-7.DOI:10.16289/j.cnki.1002-0837.2026.01001.
基金信息:
航天医学基础与应用国家重点实验室开放课题（SMFA20K30）
2026-02-25
2026-02-25