航天医学与医学工程

目的 观察益气解毒方对电离辐射致小鼠小肠功能损伤的防护效应，并探究其是否通过抑制小肠铁死亡来缓解电离辐射引起的小肠功能损伤，为航天医学中肠道辐射损伤防护药物的寻找和开发提供科学支持。方法 将378只雄性Balb/c小鼠随机分为7组：空白组、模型组、阳性药组、益气解毒方高剂量组、益气解毒方低剂量组、Liproxstatin-1照前给药组和Liproxstatin-1照后给药组，每组54只；每组又分3个批次，每批次18只。预防给药7 d后，用2.0 Gy⁶⁰Co γ射线对除空白组外的其余各组小鼠进行一次性全身照射，分别于照射后1 d、3 d、7 d观察小鼠一般情况及小肠的形态结构，检测小肠炭末推进率、血清D-木糖含量和乳酸含量，以及小肠中铁(Fe)、脂质过氧化物(LPO)、丙二醛(MDA)、谷胱甘肽(GSH)含量及超氧化物歧化酶(SOD)活力。结果 益气解毒方可以减缓2.0 Gy⁶⁰Co γ射线照射后小鼠体质量的下降程度，改善小肠的形态结构，降低小肠炭末推进率，提高血清中D-木糖含量，降低血清中D-乳酸含量；减轻小肠中线粒体的皱缩程度，降低小肠组织中Fe和MDA的含量。结论 益气解毒方可能通过抑制小肠铁死亡来缓解电离辐射引起的小肠功能损伤，为载人航天深空探测任务中肠道辐射损伤的防护提供了新策略。

Objective To observe the protective effects of Yiqi Jiedu Decoction on ionizing radiation-induced small intestinal functional injury in mice,and explore whether it alleviates such injury by inhibiting small intestinal ferroptosis,thereby providing scientific support for the discovery and development of intestinal radiation protection drugs in aerospace medicine.Methods A total of 378 male Balb/c mice were randomly divided into 7 groups:blank control group,model group,positive drug group,high-dose Yiqi Jiedu Decoction group,low-dose Yiqi Jiedu Decoction group,Liproxstatin-1 pre-irradiation administration group,and Liproxstatin-1 post-irradiation administration group,with 54 mice in each group.Each group was further divided into 3 batches,with 18 mice per batch.Seven days after preventive administration,all groups except the blank control group were subjected to a single whole-body irradiation with 2.0 Gy ⁶⁰Co γ-rays.The general condition and morphological structure of the small intestine were observed at 1,3,and 7 days post-irradiation.The small intestinal charcoal propulsion rate,serum D-xylose content,and lactic acid content were measured,along with the levels of Fe,LPO,MDA,GSH,and SOD activity in the small intestine.Results Yiqi Jiedu Decoction could mitigate the decrease in body weight of mice after 2.0 Gy ⁶⁰Co γ-ray irradiation,improve the morphological structure of the small intestine,reduce the small intestine charcoal propulsion rate,increase serum D-xylose levels,and decrease total serum lactate levels.It also alleviated mitochondrial shrinkage in the small intestine and reduced the contents of Fe and MDA in small intestine tissues.Conclusion Yiqi Jiedu Decoction may alleviate ionizing radiation-induced small intestinal functional injury by inhibiting ferroptosis in the small intestine,providing a new strategy for intestinal radiation injury in deep space exploration missions such as manned spaceflight.